Fig. 3.

Binding of anti-CD134 and Fc-TNC-CD134L to primary CD4+ T cells. Popliteal lymph node cells were stained with Fc-TNC-fCD134L, anti-CD134 and as a control for non-specific binding, Fc-TNC-hCD134. Bound ligands were detected with phycoerythrin (PE)-conjugated anti- human IgG Fc. CD134 expression was assessed using mAb 7D6, bound antibody being detected with PE-anti-mouse IgG. Cells were processed for flow cytometry and dotplots represent 10,000 events collected in LIST mode and analysis gates show percentages of total cells analysed. Dotplots are representative of two independent analyses.