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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: J Immunol. 2008 Dec 1;181(11):7670–7680. doi: 10.4049/jimmunol.181.11.7670

Figure 2.

Figure 2

Enhanced CD8 effector differentiation in TLR3−/− mice correlated with absence of immunosuppressive cytokines production after poly I:C treatment. (A) Wild type, TLR3−/− or TRIF-deficient mice were immunized with 1μg SEA with 40μg poly I:C. Five days post immunization, spleens were harvested and red blood cells lysed. One million total splenocytes were restimulated with SEA in the presence of brefeldin A for 5 hours in vitro. Intracellular staining for the presence of IFNγ and TNFα was analyzed by gating on CD4−Vβ3+ T cells. Wild type (N=6), TLR3−/− (N=13), TRIF-deficient (N=6). (B) Naïve wild type or TLR3−/− splenocytes were cultured with increasing doses of poly I:C or CpG for 24 hours in vitro. Supernatant was collected and IL-10 or IFNγ production was measured by ELISA. N=4−6. CpG treatment data is representative of 2 experiments. (C) Naïve wild type or TLR3−/− mice were i.p. injected with 40μg of poly I:C. 1, 2, or 4 hours later serum was collected and TNFα or IL-6 was measured by ELISA (N=3−4). Data presented as mean values +/− standard error of means. ** P<0.01.