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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: Ann N Y Acad Sci. 2008 Dec;1147:264–274. doi: 10.1196/annals.1427.019

Figure 4.

Figure 4

Effect of ruthenium red on mitochondrial Ca2+ homeostasis in permeabilized HeLa cells. HeLa cells were transfected with the mtAeqmut probe, permeabilized by a 1-min treatment with 25 μmol/L digitonin, and then perfused with intracellular buffer (IB)/ethylene glycol tetraacetic acid (EGTA) buffer (composition in the text). When indicated the medium was switched to IB/Ca2+. When a plateau [Ca2+]m level was reached, the perfusion medium was supplemented with (A) 10μmol/L ruthenium red (RR). The rate of Ca2+ extrusion in control cells versus (B) MitoE2- and (C) CGP37157-treated cells was then calculated. (D) When 10 μmol/L RR was added (right panel) both to IB/EGTA and to IB/Ca2+, mitochondrial Ca2+ uptake was strongly reduced as compared to nontreated cells (left panel). Note the different scales on the two panels. Traces are representative of > nine measurements.