Immunohistochemical staining of E19d rat brains with antibodies against tau phosphorylated at specific individual phosphorylation sites. Paraffin-embedded sections of E19d rat brains were immuno-stained with anti-pS199 (A), anti-pS202 (B), 12E8 (to pS262 and pS356, C), and anti-pS396 (D–F), and the immunoreaction was developed by DAB. Panels E and F are enlarged images in the area of cortex (E) and caudate putamen (F), respectively. Abbreviations: Amyg, amygdala; Cpu, caudate putamen; cpu, caudate/putamen neuroepithelium; cx, neuroepithelium; CxP, cortical plate; CxS, cortical subplate; ICx, intermediate cortical layer; LS, lateral septal nu; LV, lateral ventricle; SubV, subventricular cortical layer.