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. 2009 May 11;4(5):e5497. doi: 10.1371/journal.pone.0005497

Figure 2. Overexpression of SCC1 or SCC2 suppress the synthetic lethality between elg1 and ctf4.

Figure 2

A elg1 ctf4 double mutant maintained alive by the presence of a URA3-marked plasmid carrying the ELG1 gene was transformed with LEU2-marked plasmids overexpressing SCC1, SCC2, SMC1 or SMC3, or, as controls, with an empty vector or a plasmid carrying ELG1. Cells were successively diluted ten-fold and plated on plates lacking Uracil (SD-Ura), Leucine (SD-Leu), or on plates containing 5-fluoroorotic acid (5-FOA), which select for cells that became Ura- (i.e., lost the URA3-marked, ELG1-containing plasmid). Only plasmids overexpressing SCC1 and SCC2 allowed cells to grow on 5-FOA plates.