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. 2009 May 11;4(5):e5497. doi: 10.1371/journal.pone.0005497

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Parnas et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Whole cell extracts from cells at mid-logarithmic growth phase were fractionated into a chromatin and a soluble fraction, and the presence of Myc- tagged Elg1 was followed with anti-Myc specific antibodies. As controls, anti-Carboxypeptidase Y and anti- Acetylated histone H4 antibodies were used. (A) Elg1 is present in both fractions, but its abundance decreases with DNA damage. Cells were untreated, or incubated in the presence of methyl methanesulfonate (MMS) at two different concentrations (0.02% and 0.3%). (B) Ctf18 is present in both fractions, and its localization in G2 depends on Elg1. Wild type or elg1 cells were arrested with either alpha factor (G1), hydroxyurea (early S) or nocodazole (G2) before being fractionated.