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. 2009 Mar 31;100(8):1320–1329. doi: 10.1038/sj.bjc.6605010

Figure 1.

Figure 1

12-0-Tetradecanoylphorbor-13-acetate (TPA)-dependent ectodomain shedding induces phosphorylation of both EGFR and MAPK in MKN45 cells. (A) MKN45 cells were treated with 200 nM TPA for 0, 15, 120, and 240 min respectively. The endogenous phospho-EGFR and phospho-p44/42 MAPK, along with total EGFR and total p44/42 MAPK expression levels, were analysed by western blotting. (B) Before the treatment of MKN45 cells with 200 nM TPA for indicated periods, the cells were preincubated with 1 μM AG1478 (an EGFR tyrosine kinase inhibitor) and 20 μM U0126 (an MEK 1/2 inhibitor) for 60 min. The endogenous phospho-EGFR and phospho-p44/42 MAPK, along with total EGFR and total p44/42 MAPK expression levels, were analysed by western blotting.