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. 2009 Mar 17;100(8):1347–1357. doi: 10.1038/sj.bjc.6604985

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Copyright © 2009 Cancer Research UK

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Effect of Bcl-2 associated athanogene (BAG-1)S overexpression on transcriptional regulation by p53 family proteins. (A) SaOs2 cells were transfected with 100 ng of Bax-luc reporter construct and 100 ng of p53, 100 ng of p63 or 50 ng of p73α expression plasmids, respectively, in the presence or absence of BAG-1S expression construct (2500 ng). Transfected cells were analysed for luciferase activity (top) and BAG-1S expression (by immunoblotting (IB); bottom) 48 h after transfection. Data shown are the mean luciferase activity (±s.d.) of duplicate transfections normalised to cells transfected with Bax-luc only (set to 1.0). (B) SaOs2 cells were transfected with 100 ng of Bax-luc reporter construct in the absence or presence of p73α expression plasmid (50 ng) and increasing amount of BAG-1S expression construct (0, 500, 1000 ng). Transfected cells were analysed for luciferase activity (top) and BAG-1S expression (bottom) 48 h after transfection. Data shown are the mean luciferase activity (±s.d.) of duplicate transfections normalised to cells transfected with Bax-luc only (set to 1.0). (C) SaOs2 cells were transfected with 200 ng of pGL-Basic or Bcl-XIB reporter constructs in the absence or presence of 1000 ng of BAG-1S expression plasmid and luciferase activity (top), and BAG-1S expression (by immunoblotting (IB); bottom) was measured after 48 h. Data shown are the mean luciferase activity (±s.d.) of duplicate transfections normalised to cells without BAG-1S overexpression (set to 1.0). The experiments shown in (A–C) are representative of two similar experiments.