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. Author manuscript; available in PMC: 2010 Feb 27.
Published in final edited form as: Chem Biol. 2009 Feb 27;16(2):141–147. doi: 10.1016/j.chembiol.2009.01.007

Table 1.

Deduced functions of orfs in the thiostrepton biosynthetic gene cluster

Gene Sizea Protein Homologb and Origin Identity/Simiarity, % Proposed Function sio Homolog tcl Homolog
orf1c 193 Upp (NP_628223), from Streptomyces coelicolor A3(2) 91/97 uracil phosphoribosyltransferase
orf2c 208 SGR_3903 (YP_001825415), from S. griseus subsp. griseus NBRC 13350 41/53 hypothetical protein
orf3c 177 SSCG_02932 (YP_002192918), from S. clavuligerus ATCC 27064 68/84 hypothetical protein
orf4c 142 MesJ (NP_696675), from Bifidobacterium longum NCC2705 57/68 cytidine and deoxycytidylate deaminase
tsrA 362 HisC2 (YP_001853585), from Mycobacterium marinum M 44/60 histidinol-phosphate aminotransferase
tsrB 276 SSEG_03719 (YP_002206063), from S. sviceus ATCC 29083 50/65 predicted hydrolase of the alpha/beta superfamily
tsrC 618 OxyD (AAZ78328), from S. rimosus 54/65 amidotransferase
tsrD 135 SnoaL (AAF01813), from S. nogalater 32/54 polyketide clyclase-like enzyme
tsrE 428 STH2357 (YP_076186), from Symbiobacterium thermophilum IAM 14863 35/51 acyl-CoA dehydrogenase-like protein
tsrF 311 Sare_0495 (YP_001535415), from Salinispora arenicola CNS-205 41/57 putative methyltransferase
tsrG 213 Mext_1123 (YP_001638598), from Methylobacterium extorquens PA1 28/46 hypothetical protein
tsrH 58 ---------- --/-- thiostrepton precursor peptide sioH tclB1–4
tsrI 197 Bm1_20485 (XP_001895551), from Brugia malayi 29/42 hydrolase/esterase
tsrJ 933 SpaB (AAL15564), from Bacillus subtilis 11/24 dehydratase sioJ tclE
tsrK 346 SpaB (AAL15564), from B. subtilis 12/29 dehydratase sioK tclF
tsrL 368 BC5082 (NP_834750), from B. cereus ATCC 14579 21/41 hypothetical protein sioL tclG
tsrM 589 BAT_3535 (ZP_03054696), from B. pumilus ATCC 7061 24/40 dehydrogenase sioM tclH
tsrN 612 BL02837 (YP_078248), from B. licheniformis ATCC 14580 25/39 hypothetical protein sioN tclK
tsrO 681 PatD (AAY21153), from Prochloron didemni 22/35 cyclodehydratase sioO tclD
tsrP 474 PimD (CAC20932), from S. natalasis 26/42 P450 epoxidase
tsrQ 424 PstD (CAM56771), from Actinoplanes friuliensis 29/39 acyl-CoA synthetase
tsrR 396 RubU (AAM97370), from S. collinu 30/44 cytochrome P450 monoxygenase
tsrS 936 SpaB (AAL15564), from B. subtilis 11/23 dehydratase sioS
tsrT 599 Gura_3608 (YP_001232335), from Geobacter uraniireducens Rf4 27/39 radical SAM domain-containing protein
tsrU 272 FabG (YP_177255), from B. clausii KSM-K16 36/51 dehydrogenase/reductase
orf5c 247 orf_L7 (CAN89623), from S. collinus 39/60 hypothetical protein
orf6c 1447 SsviA_010100017073 (ZP_03195299), from S. sviceus ATCC 29083 38/51 WD-40 repeat protein
orf7c 99 SCO4036 (NP_628218), from S. coelicolor A3(2) 82/91 hypothetical protein
orf8c 336 SclaA_010100013725 (ZP_03183088), from S. clavuligerus ATCC 27064 79/81 RNA polymerase sigma factor, sigma F
a

Numbers are in amino acids.

b

NCBI accession numbers are given in parentheses.

c

orfs beyond the tsr gene cluster