Table 1.

Tumor growth and CTL induction after transfer of MC-GP cell suspension or tumor pieces in C57BL/6 or ALY × ALY mice

Transferred cells, dose	Recipient	Tumor growth*		CTL priming†
		Growing	Tumor free	Growing tumors, CTL+/total tested	No tumors, CTL+/total tested
MC-GP (2 × 106) Cell suspension	C57BL/6	0	30	Not testable	15/15
MC-GP (2–5 × 106) Tumor piece	C57BL/6	119	41	0/22	10/15‡
MC-GP (107) Cell suspension	ALY × ALY	6	0	0/6	Not testable
MC-GP (2–5 × 106) Tumor piece	ALY × ALY	6	0	0/6	Not testable

^*Tumor size was followed for 4 weeks or longer. Tumors reaching a volume of 1 cm³ or more were counted as growing tumors. 

^†CTL activity was determined at day 8 after injection of cell suspensions or 4 weeks after transplantation of solid tumor pieces after in vitro stimulation with GP33-41-pulsed splenocytes during 5 days; the numbers shown represent number of mice responding with secondary CTLs (CTL⁺) over total number of mice tested in each category. 

^‡Five transplanted tumor pieces did not grow because of transplantation failure, depending on the quality of the transplanted tumor pieces.