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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: Biochim Biophys Acta. 2007 Feb 22;1773(6):784–793. doi: 10.1016/j.bbamcr.2007.02.004

Fig. 1.

Fig. 1

Multiple Rab proteins are on the surface of lipid filled adiposomes. Purified droplets were processed either for immunoblotting (IB) or immunogold localization of the indicated Rab protein (Immunogold). For immunogold, samples were fixed and incubated in the presence of rabbit IgG (A), pAb α-Rab5 (B), pAb α-Rab11 (C), or pAb α-Rab18 (D). The samples were then incubated with the α-rabbit IgG conjugated to 10 nm gold. Gold particles (arrows) are distributed on the surface of the droplet. An asterisk indicates clusters of small droplets. Immunoblotting shows that each antibody recognizes a single band. Bar, 0.5 μm.