Langerhans dendritic cell (DC) trafficking during edema. In normal skin, some DCs (red) were seen between the epidermis and lymphatic vessels (green) in the skin, indicating normal migration. After 7 days of edema, no DCs were found below the epidermis. At 14, 21, and 30 days, DCs were present in high numbers in the dermis and near the hyperplastic lymphatic vessels, indicating migration. Yellow arrows indicate some DCs in the epidermis, and white arrows indicate migrating DCs. Scale bar=200 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)