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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: J Neurovirol. 2006 Dec;12(6):492–500. doi: 10.1080/13550280601064798

Figure 2.

Figure 2

MOR agonists inhibit CXCL12-mediated Akt and ERK phosphorylation in cortical neurons. The effect of DAMGO (1 or 10 μM) or endomorphin-1 (100 nM) on Akt and ERK1/2 phosphorylation induced by CXCL12 (20 nM, 15 min) was studied by Western blot. A general opioid receptors antagonist, naloxone (NALX, 50 μM) (A, B), or a MOR-selective antagonist, CTAP (1 μM, D) was used to determine receptors involved. After blotting with phosphospecific antibodies, membranes were stripped and reblotted using antibodies against total Akt or total ERK1/2. Graphs report average ± SEM of P-Akt or P-ERK band density from three independent experiments. ** P < .001 versus control; ˆˆP < .001 versus CXCL12 alone.