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DAMGO transiently phosphorylates Akt and ERK in SH-SY5Y neuroblastoma cells. Acute DAMGO treatment phosphorylates Akt and ERK in SH-SY5Y cells, in a time- (A, 5–60 min; B, 1–10 min) and dose (C, 10 nM to 10 μM) dependent manner. Peak activation occurs at 3 to 5 min. After blotting wit phosphospecific antibodies, membranes were stripped and reblotted using antibodies against total Akt or total ERK1/2.
