Figure 3. HIF-1 modulates lifespan extension by DR.

(A–E) Survival curves and (F) mean lifespan of N2, hif-1 and egl-9 animals fed with E. coli at different concentrations (1.0×10⁸ to 1.0×10¹² cfu/ml) during adulthood. The hif-1 mutant shows lifespan extension under AL and higher bacterial concentrations but not under DR. egl-9 animals show diminished lifespan extension under DR, but they show lifespan similar to that of N2 animals under AL conditions. Each of the lifespan experiments was performed three times with consistent results. Quantitative data and statistical analyses for the experiments shown here and the repeated experiments are included in Table 2 and Table S2, respectively.