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. 2009 May 22;5(5):e1000486. doi: 10.1371/journal.pgen.1000486

Figure 6. HIF-1 functions through the IRE-1 ER stress pathway to modulate lifespan.

Figure 6

(A) IRE-1 modulates lifespan extension by DR. ire-1(v33) mutant animals show significantly reduced changes in lifespan upon nutrient manipulation. (B) ire-1(v33) fully suppresses lifespan extension by hif-1 under both AL and DR conditions. Each of the lifespan experiments was performed multiple times with consistent results. Quantitative data and statistical analyses for the experiments shown here are included in Table 2 and Table 4. Analyses for the repeated experiments are included in Table S2 and Table S5. (C) hsp-4 mRNA levels are regulated by DR and HIF-1. DR reduces hsp-4 transcription in N2, hif-1 and egl-9 (p<0.05, t-test). Overexpression of HIF-1 due to the egl-9(sa307) mutation results in increased hsp-4 transcription under both AL and DR conditions (p<0.01, t-test). qRT-PCR experiments were performed twice with consistent results using two independent RNA preparations.