Figure 4.

Decrease in IL-3 expression in intestine of diabetic rats and restoring effect by insulin and RU 486. Animals were treated with insulin (3 IU, s.c.) or RU 486 (20 mg/kg, p.o.) once a day for 18 days following diabetes induction. Panels (a) and (b) show photomicrographs of immunostaining of IL-3 of intestine sections from alloxan-diabetic rats. Panels (c) and (d) show photomicrographs of immunostaining of IL-3 of intestine sections from insulin-treated diabetic rats and diabetic rats treated with RU 486 respectively. Insets represent negative controls when an irrelevant biotinylated goat anti-hamster IgG was used as primary antibody. Sections were counterstained with Harris haematoxylin. Bar 0.1 μm. Quantitative evaluations of IL-3 labelling after insulin and RU 486 treatment are seen in panels (e) and (f) respectively. Data are expressed as mean ± SEM from four animals. The data are representative of two experiments. *P < 0.05 compared with alloxan-diabetic rats.