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RAG expression in Ras-JHΔ B lineage cells. Reverse transcription–PCR analyses of first-strand cDNA derived from sorted populations of peripheral B220+ Ly 9.1+ Ras-JHΔ (Ras-JH−/−) and mature wild-type B cells (WT B), together with purified bone marrow B220+ CD43− IgM− wild-type pre-B cells (WT Pre-B) are shown. Serial five-fold dilutions of cDNA are depicted, and ES cell cDNA was diluted into the 5- and 25-fold dilutions in each sample set to equalize template quantity.
