Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis of λ LC gene rearrangement in Ras-JHΔ B lineage cells. PCR analysis was performed on DNA isolated from B220+ Ly 9.1+ Ras-JHΔ (Ras-JH−/−) and wild-type peripheral B lineage cells purified by cell sorting of lymph node and spleen. A titration of B cell DNA is shown after Southern blot analysis using a germline Vλ1 probe that cross-hybridizes to Vλ2; the total amount of DNA was held constant at 25 ng in each sample through the addition of murine embryonic fibroblast (MEF) DNA.
