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. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: Toxicol Lett. 2008 Dec 30;185(3):180–186. doi: 10.1016/j.toxlet.2008.12.014

Figure 2.

Figure 2

Western blot analysis of Nrf2 protein in HepG2 cells. HepG2 cells were treated with vehicle (control, ctrl., DMSO 0.1% v/v), or 25 μM of tBHQ, oltipraz (olti.), coumarin (coum.), limettin (lim.), auraptene (aurap.), imperatorin (imper.) and isopimpinellin (isop.) for 24 h. Whole cell lysates were immunoblotted for Nrf2 and compared to β-actin as a loading control. The lower panel shows the ratio of the integrated density of Nrf2:actin.