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. Author manuscript; available in PMC: 2010 Feb 11.
Published in final edited form as: Vaccine. 2008 Dec 9;27(7):1136–1140. doi: 10.1016/j.vaccine.2008.11.071

Figure 3.

Figure 3

Representative plots of antigen-specific CD8+ T cells in a vaccine responder. PBMC were stimulated with Env or CMV peptides then stained with anti-CD107a PE, anti-IFN-γ FITC, anti-TNF-α PE-CY7, anti-CD4 PerCP-Cy5.5 anti-IL-2 APC, anti-CD3 AmCyan and anti-CD8 APC-Cy7, and analyzed by flow cytometry. Samples were first gated on the CD3+/CD8+ lymphocyte population then the percent of CD107, TNF-α, IFN-γ, and IL-2 positive CD8+ T cells were determined.