FIGURE 3.

Expression of I-A and costimulatory molecules on CD11b⁺CD11c⁺ DC, CD8α⁺CD11c⁺ DC subsets of EAE and i.v. tolerized mice. A, Flow cytometric analysis of cell surface markers on freshly isolated MNCs. Splenocytes of EAE mice or MOG_35–55 i.v. tolerized mice were isolated on day 21 p.i. and stained for the expression of I-A, CD86, CD80 and CD40 on CD11c⁺CD11b⁺ DCs and CD11c⁺CD8α⁺ DCs.*, p < 0.05;**, p < 0.01;***, p < 0.001. B, Flow cytometric analysis of surface markers on freshly isolated CNS cells from PBS i.v. EAE mice or MOG_35–55 i.v. tolerized mice. Cells were stained for expression of I-A, CD86, CD80, and CD40 on CD11c⁺CD11b⁺ and CD11c⁺CD8α⁺ DC subsets. Blue histogram lines represent specific fluorescence for indicated cell surface markers and red histogram lines represent cells stained with isotype-matched control mAbs.*, p < 0.05;**, p < 0.01;***, p < 0.001. One representative experiment of three is shown.