Fig.7.

Hypotonic solution caused rapid swelling in both WT and Cx43-deficient astrocytes monitored by intracellular dye dilution. (A) Confocal images of calcein loaded WT astrocytes before, during and 2 minutes after exposure to hypotonic solution, note decrease in calcein fluorescence in the presence of hypotonic solution, indicating cell swelling. (B and C) Hypotonic solution produced similar decreases in calcein fluorescence in normal and Cx43 KO astrocytes during brief exposure to hypotonic solution. scale bar = 10 µm.