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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: Leukemia. 2007 Jun 7;21(8):1752–1762. doi: 10.1038/sj.leu.2404798

Figure 3.

Figure 3

NF-κB binding activity on a consensus κB binding site in various cell lines. (a) Approximately 5 µg of nuclear lysates from the cells were incubated with a [32P]-radiolabeled oligonucleotide corresponding to MHC class 1 gene κB sequence and analyzed by EMSA. The complexes are indicated as C1, C2 and C3. (b) In supershift assays, antibodies specific for each NF-κB subunit (indicated above the lane) were incubated with the nuclear extracts from MT-2, RV-ATL, HT-1RV and IMM-1 cells before addition of the probe.