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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: Leukemia. 2007 Jun 7;21(8):1752–1762. doi: 10.1038/sj.leu.2404798

Figure 6.

Figure 6

PTHrP P2 promoter can be transactivated by NF-κB in a subunit-dependent manner: Relative luciferase activity in NIH3T3 cells transfected with (a) 1µg of either wild-type pGL2/PTHrP-P2/Luc, mutant pGL2/PTHrP-P2/Luc or pGL2 constructs in the presence or absence of expression vectors for NF-κB p50, p65, c-Rel, Bcl-3, p50/p65, p50/c-Rel or p50/Bcl-3 or Tax; (b) 1µg wild-type pGL2/PTHrP-P2/Luc with or without 0.5 µg of NF-κB c-Rel expression vector and 0.1–0.5 µg of p50 expression vector; (c) 1µg wild-type pGL2/PTHrP-P2/Luc with or without 0.5 µg of NF-κB p50 expression vector and 0.05–0.25 µg of c-Rel expression vector; (d) 1µg wild-type pGL2/PTHrP-P2/Luc with or without 0.2–0.5 µg of NF-κB p50 expression vector and 0.25–0.5 µg of Bcl-3 expression vector; (e) 1µg wild-type pGL2/PTHrP-P2/Luc with or without 0.05–0.5 µg of NF-κB Bcl-3 expression vector and 0.5 µg of p50 expression vector. The quantity of the expression plasmids is indicated in µg. Bars represent the mean ± s.d. of three independent samples. (**) indicates significant differences between various groups compared to PTHrP P2 Luc alone (P<0.05). (*) indicates significant differences between mutant and wild-type (P<0.05).