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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Clin Cancer Res. 2009 Jan 1;15(1):100–109. doi: 10.1158/1078-0432.CCR-08-1745

Figure 7. PMIP is associated with reduced Muc1 expression.

Figure 7

a) A representative MMTV- pyV mT msPMIP (P) treated mouse and a PTD4 mouse (C) were each injected 30 minutes prior to sacrifice with epidermal growth factor (1µg/g body weight) and with peptide. Following sacrifice the tumors were collected and protein lysates were generated. Protein (50µg) was separated by SDS-PAGE, transferred and immunoblotted for expression of phosphotyrosine (PY99), EGFR (1005), Muc1 (CT2), and β-actin (AC-15). b) Lysates from MDA-MD-231 xenograft tumors (not treated with EGF; described in Figure 2) were similarly analyzed to determine levels of EGFR and MUC1 protein expression. Relative protein levels of Muc1 were measured by densitometry and graphed (Muc1/β-actin, *, p=0.014, ANOVA). Molecular weights are shown on the right. IB = immunoblot. White lines through blots indicate same gel and exposure but were non- contiguous.