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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):2583–2589. doi: 10.4049/jimmunol.0803247

Figure 3.

Figure 3

Panel A: Immunoblot of FcRn protein in the mouse yolk sac. Tissue lysates of equal protein concentration from samples of yolk sac (YS), placenta (Plac), neonatal intestine (Int) from FcRn+/+ and FcRn−/− conceptuses were analyzed by SDS-PAGE and immunoblot (IB) with rabbit anti-rat FcRn antibody. Panel B: Mobility of de-glycosylated FcRn from yolk sac and neonatal intestine. The lysates were equilibrated with the appropriate buffer, incubated with or without N-glycosidase F(+/− N-gly F), and analyzed by immunoblotting with the rabbit anti-rat FcRn antibody. Numbers are MW markers in kDa.