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Effect of macromolecular crowding agent Ficoll-70000 (150 mg/ml, dashed lines) on near-UV CD (A) and far-UV CD spectra of human insulin (B) in different conformational states: native hexameric protein in 25 mM HEPES buffer, pH 7.5 (black lines) and in 20% acetic acid, pH 2.0 (gray lines). Solid lines in both plots correspond to spectra measured in the absence of the crowder.
