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. Author manuscript; available in PMC: 2009 Aug 26.
Published in final edited form as: Biochemistry. 2008 Jul 30;47(34):8993–9006. doi: 10.1021/bi8008399

Figure 7.

Figure 7

Fibrillation of human recombinant α-synuclein in the absence (black symbols) or presence of 100 mg/ml PEG-3500 (open symbols). Protein was incubated alone (circles) or in the presence of 1 (reversed triangles), 2 (squares) or 2.5 M TMAO (diamonds). Panel A represents a set of normalized data, whereas Panel B shows the same data in the non-normalized form. In Panel B numbers correspond to: 1, control, no additives; 2, α-synuclein + PEG; 3, 1 M TMAO; 4, 1 M TMAO + PEG; 5, 2 M TMAO; 6, 2 M TMAO + PEG; 7, 2.5 M TMAO; 8, 2.5 M TMAO + PEG. Protein at concentration of 0.5 mg/ml was incubated in 20 mM Tris-HCl, 100 mM NaCl buffer pH 7.5 at 37°C with agitation and fibrillation was monitored by the enhancement of thioflavin T fluorescence intensity.