Skip to main content
. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Cell Signal. 2008 Aug 23;20(12):2221–2230. doi: 10.1016/j.cellsig.2008.08.011

Fig. 3.

Fig. 3

SOCS3-deficient cells fail to upregulate p21 mRNA after irradiation. Approximately 1×107 WT or SOCS3 knockout MEFs were irradiated as described in Fig. 1. Messenger RNA was isolated at the indicated time points and mRNA levels for several cyclin and p53-dependent genes were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results were quantitated by densitometry and are expressed as mean±SEM. Statistical analysis was performed using the Students t-test. *p<0.01, **p <0.05, n=3.