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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Cell Signal. 2008 Aug 23;20(12):2221–2230. doi: 10.1016/j.cellsig.2008.08.011

Fig. 5.

Fig. 5

Proximal events in the DNA damage response are unaffected by SOCS3-deficiency. Approximately 1×107 WT or SOCS3-deficient MEFs were irradiated as in Fig. 1. (Panel A) Protein extracts were processed at the indicated time points, followed by Western blotting for phosphorylated p53 (top panel) or total p53 protein (bottom panel). (Panel B) Irradiated cells were analyzed for phosphorylated H2AX by flow cytometry as described in the Materials and methods. Results are expressed as the fold increase in H2AX mean fluorescence intensity (MFI) in irradiated:unirradiated controls.