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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: Biochemistry. 2006 Dec 19;45(50):15075–15084. doi: 10.1021/bi061279k

Figure 3. Sse1 and bHsp70ΔC form a protease-resistant complex.

Figure 3

The indicated purified proteins were incubated at a concentration of 10 μM with a series of increasing concentrations of exogenously added proteinase K (grey triangle) ranging from 0 to 16 μg/ml for 30 min and digestion reactions terminated by the addition of PMSF to 1 mM. Digestion products were resolved on a 8-25% SDS-PAGE gel and Coomassie-stained. A, Sse1 alone, B, Sse1 with 1 mM ATP, C, Sse1 and bHsc70ΔC with 1 mMM ATP, D, Sse1 and bHsc70ΔC with no ATP, E, Sse1 and the bHsc70 NBD with 1 mM ATP.