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. Author manuscript; available in PMC: 2009 May 4.
Published in final edited form as: Biochemistry. 2006 Dec 19;45(50):15075–15084. doi: 10.1021/bi061279k

Figure 4. The Sse NBD dictates binding specificity and is necessary and sufficient for interaction with Ssa.

Figure 4

A, Serial dilutions of sse1Δ cells expressing the indicated Sse1-Sse2 chimeras or empty vector were spotted onto plates and incubated at 30°C for 2 days. B, FLAG-tagged versions of the indicated Sse1-Sse2 chimeras (described in Experimental Procedures) were expressed in sse1Δ yeast and immunoprecipitated from protein extracts. Protein complexes were resolved by SDS-PAGE and analyzed by Coomassie staining or anti-Sse1 immunoblotting. C, sse1Δ cells expressing FLAG-Sse1 (lane 1), FLAG-Sse1 NBD (lanes 2 and 3), FLAG-Sse2 NBD (lanes 4 and 5) or Sse1 PBD (lanes 3 and 5) were subjected to FLAG immunoprecipitation. Protein complexes were resolved by SDS-PAGE and stained with Coomassie.