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. 1999 Mar 2;96(5):2256–2261. doi: 10.1073/pnas.96.5.2256

Figure 1.

Figure 1

Effect of IL-18 on IFN-γ induction. (A) Enhancing effect of mature IL-18 (10 nM) on phytohemagglutinin- (10 μg/ml) induced production of IFN-γ in human PBMCs. ProIL-18 was also added at 10nM. PBMCs were stimulated for 72 h, and IFN-γ was assayed on supernatants by ECL assay. Data are the mean ± SEM (n = 3). (B) Effect of reversible ICE inhibitor (ICEi) and IL-1Ra on LPS-induced IFN-γ production in whole human blood. Whole blood was stimulated with LPS (10 ng/ml) in the presence of ICEi (10 μM) or IL-1Ra (10 μg/ml) and after 24 h, the concentration of IFN-γ was measured in the whole blood after lysis in 0.5% Triton X-100. Data are the mean ± SEM (n = 3). A paired Student’s t test was used to assess significance between LPS and LPS + ICEi.