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. 1999 Mar 2;96(5):2256–2261. doi: 10.1073/pnas.96.5.2256

Figure 2.

Figure 2

Effect of ICE inhibitor on IL-1β and IL-18 processing and release. PBMCs from two separate donors were stimulated with LPS (100 ng/ml) in the presence or absence of 10 μM ICE inhibitor (ICEi). The letter C above the lanes indicates the unstimulated control PBMCs. After 24 h at 37°C, the supernatants were removed and concentrated in a Centricon device, electrophoresed in two separate gels, and blotted (in duplicate). (A) Western blotting with anti-human IL-1β. (B) Duplicate blot to that in A but developed with anti-human IL-18. (C) Cell lysates from the same cultures used in A but developed with anti-human IL-1β. (D) Same cultures as used in A but developed with anti-human IL-18. Molecular mass makers (in kDa) are indicated by arrows on left side of figure. r-pIL-18 and r-mIL-18 indicate recombinant pro- and mature IL-18, respectively.