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Detection of IL-18 in freshly isolated human PBMCs. Whole blood from three donors was obtained and PBMCs were isolated. Without further processing, cells were counted and centrifuged at 250 × g, and lysis buffer was immediately added. The lysates were subjected to SDS/PAGE with 15% gels and Western blotting with horseradish peroxidase-conjugated anti-rabbit IgG. Recombinant human pro- and mature IL-18 are indicated as in Fig. 2. Each lane represents a different human donor.
