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Effect of IFN-γ on time course of LPS-induced expression of IL-12 and IL-18 (A) IL-12. PBMCs were stimulated with LPS (100 ng/ml) with and without IFN-γ (250 units/ml). Lanes: 1, unstimulated PBMCs; 2, IFN-γ; 3 and 6, after 1 h; 4 and 7, after 3 h; 5 and 8, after 6 h. (B) IL-18. Lanes: 1, unstimulated PBMCs; 2 and 6, after 1 h; 3 and 7, after 3 h; 4 and 8, after 6 h; 5 and 9, after 24 h.
