Different stages of plant transformation, nuclear stable (A–F) and viral transient (G–I). (A) Selection of explants after co-cultivation with A. tumefaciens, (B–D) calli formation, (E) regeneration of whole transgenic plants, (F) generation of stable transgenic cell suspension culture (G–H) A. tumefaciens mediated viral infection in contained greenhouse environment, (I) expression of GFP reporter gene after 10 days post infection.