Fig. 4. Effect of CD56+ T supernatant (SN) on susceptibility of hepatocytes to HCV infection.
Huh 7.5.1 cells (A) or primary human hepatocytes (B) were cultured in the presence or absence of CD56+ T SN (50%, v/v) for 24h prior to HCV JFH-1 infection. Total cellular RNA was extracted from the cell cultures at the indicated time points and subjected to the real-time RT-PCR for HCV and GAPDH RNA quantification. The data are expressed as log HCV RNA copies/μg RNA. The results shown are mean ±SD of triplicate cultures, representative of three experiments. UD: undetectable.