Table 3. Down-Regulated Genes in JAK/STAT Pathway by CD56+ T Supernatant.
Gene array analysis of CD56+ T SN-regulated genes in JAK/STAT pathway in primary human hepatocytes Primary human hepatocytes were incubated with or without CD56+ T SN (50%, v/v) for 6h. Total RNA was harvested and subjected to the gene array using RT2 ProfilerTM PCR Array System (SuperArray Bioscience Corporation). Data analysis was performed using Data-Analysis-Template from Superarray Bioscience Corporation. The genes which displayed less than 2-fold up- or down-regulation relative to that of the control group were omitted. The CD56+ T SN up-regulated about 24 genes (Table 2) and down-regulated 10 genes (Table 3) over 2-fold in JAK/STAT pathway.
| Gene | Description | Fold |
|---|---|---|
| PRLR | Prolactin receptor | -5.62 |
| F2R | Coagulation factor II (thrombin) receptor | -2.89 |
| SH2B1 | SH2B adaptor protein 1 | -2.81 |
| BCL2L1 | BCL2-like 1 | -2.55 |
| PIAS1 | Protein inhibitor of activated STAT, 1 | -2.53 |
| GHR | Growth hormone receptor | -2.43 |
| SIT1 | Signaling threshold regulating transmembrane adaptor 1 | -2.41 |
| INSR | Insulin receptor | -2.39 |
| CSF1R | Colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog |
-2.14 |
| SRC | V-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
-2.04 |