Figure 2.

V_HQ52 expression is enriched among intermediate PtC-binders. The FACS phenotype of individual BALB/c PtC-binding cells is overlaid on the FACS plot for the B cell population from which the cells were sorted. The PtC-binding cells were sorted from a pool of 9 BALB/c mice. The three most frequently expressed V_H families (Q52, V_H11, and V_H12), are displayed separately from other V_H families (V_HJ558, V_H10, V_H3660, V_H7183). Specialized FACS hardware and software developed at Stanford were used to determine the precise phenotype of cells that were sorted and subsequently analyzed by single-cell RT-PCR and sequencing.