Figure 3.

J_H and D gene usage differ for the four most frequently expressed V_H families among anti-PtC antibodies. Analyses shown are based on unique sequences combined from all three strains of mice (BALB/c, C57BL/6, C.B-17). Data are expressed as percentage of cells sequenced within each V_H family. Some D segments are at times indistinguishable from each other and are therefore grouped; several could not be identified and are grouped as “???”. Reading frame usage is not significantly different among the V_H families (not shown). There is no significant difference between V_H12 and V_H11 for J_H usage (Kruskal–Wallis, P = 0.09). J_H usage of V_H11 and V_H12 significantly differs from V_HJ558 and V_HQ52 (Kruskal–Wallis, P < 0.0002 for all combinations). The usage of V_HQ52 and V_HJ558 significantly differ from one another (P = 0.02).