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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Hepatology. 2009 May;49(5):1695–1708. doi: 10.1002/hep.22839

Figure 6.

Figure 6

Immunoprecipiation (IP) of SOCS1 but not SOCS3 with TIRAP in primary isolated C57BL/6 hepatocytes at time points up to 24h after treatment with LPS (A). Immunoprecipitates were immunoblotted with anti-TIRAP, SOCS1 or SOCS3 antibodies, using whole cell lysates from hepatocytes treated with 100ng/mL LPS for 8h as a positive control (A). Protein expression by Western blot of TIRAP in C57BL/6 hepatocytes treated with LPS for up to 48h (B, upper images), and actin loading controls. Similar groups of C57BL/6 hepatocytes were pretreated for 24h with adenovirus expressing control vector (AdΨ5) or SOCS1 (AdSOCS1) and then stimulated for up to 24h with LPS. Whole cell lysates were then immunoblotted with anti-TIRAP antibody and actin as a loading control (B, lower images). TIRAP ubiqitination in C57BL/6 hepatocytes at time points up to 24h after stimulation with 100ng/mL LPS, using total ubiquitin as a positive control (C). All results are representative of at least three separate experiments using hepatocytes from separate mice.