Fig. 7.

Cholinergic Ca²⁺ transients in guinea-pig smooth muscle cells. a Effect of neostigmine and 4-DAMP on spontaneous Ca²⁺ transients. Histogram showing the number of spontaneous Ca²⁺ transients recorded in guinea-pig vas deferens smooth muscle cells, with no pulse, 1 pulse and 50 pulses at 10 Hz, in control, in the presence of neostigmine (10 μM) and blocked by 4-DAMP (10 nM). b Spontaneous Ca²⁺ transients. 1 Control. 2 In the presence of neostigmine (shown by green arrows). 3 Blocked by 4-DAMP. c Evoked Ca²⁺ transients in the presence of prazosin and α,β-MeATP by trains of 50 pulses at 10 Hz; Ca²⁺ wave travelling along vas deferens preparation