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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Microbiology (Reading). 2008 Oct;154(Pt 10):3199–3211. doi: 10.1099/mic.0.2008/017319-0

Fig. 6.

Fig. 6

Transcriptional analysis of the ebpAfm to bpsfm gene cluster. (a) Northern hybridization of total RNA (30 μg/lane) from TX0082 probed with intragenic fragments of ebpAfm, ebpBfm, ebpCfm, and bpsfm. The acm probe was used as a control. RNA bands with expected sizes are indicated with arrows. (b) RT-PCR analysis of the ebpAfm to bpsfm gene cluster. Location of each primer pair is shown with arrows in the schematic representation of the gene cluster. A lollypop between ebpCfm and bps depicts a predicted transcriptional terminator. Top gel, RT-PCR with DNase-treated total RNA (10 ng) as template; middle gel, control reaction of the same total RNA (100ng) preparation amplified without reverse transcriptase; bottom gel, control reaction amplified with genomic TX0082 DNA as template. An intragenic region of gyrase A was used as a control. M, molecular weight marker.