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. Author manuscript; available in PMC: 2009 May 5.

Published in final edited form as: Angiogenesis. 2008 May 27;11(3):301–310. doi: 10.1007/s10456-008-9113-5

CAMs were treated with recombinant sphingomyelinase D (SMaseD) and the number of vessels that intersected the disc was counted. This enzyme is able to induce an angiogenic response (5A) similar to the LPA response. Two catalytically inactive forms of this enzyme (H37N and H73N) were unable to induce angiogenesis (5B), suggesting that the response obtained is dependent on the catalytic activity (lyso-PLD) of the arachnid enzyme. Moreover, SMaseD-induced angiogenesis is blocked by the LPA receptor antagonist VPC32183 at 10µM (5C), suggesting that the positive response obtained is dependent on the activation of LPA_1,3 receptors. One-Way ANOVA test was performed between groups. ** P< 0.001. Error bars represent standard deviation (SD).

CAMs were treated with recombinant sphingomyelinase D (SMaseD) and the number of vessels that intersected the disc was counted. This enzyme is able to induce an angiogenic response (5A) similar to the LPA response. Two catalytically inactive forms of this enzyme (H37N and H73N) were unable to induce angiogenesis (5B), suggesting that the response obtained is dependent on the catalytic activity (lyso-PLD) of the arachnid enzyme. Moreover, SMaseD-induced angiogenesis is blocked by the LPA receptor antagonist VPC32183 at 10µM (5C), suggesting that the positive response obtained is dependent on the activation of LPA_1,3 receptors. One-Way ANOVA test was performed between groups. ** P< 0.001. Error bars represent standard deviation (SD).