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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Exp Cell Res. 2008 Mar 20;314(10):2110–2122. doi: 10.1016/j.yexcr.2008.03.008

Figure 1. Growth of cancer cell lines and fluorescent clones as spheroids in serum-free medium (SFM).

Figure 1

A. Left panel: Phase contrast and fluorescence micrographs of spheroids after 7 day-culture of cancer cells in SFM. GL-261, EMT6, 4T1 and FEMX-1 cancer cell lines were transduced with SF91/DsRed2 or SF91/eGFP retroviral vectors and cloned by limiting dilution before being cultured in SFM. Micrographs were obtained with a Zeiss Axiovert 200 M microscope and Axiovision software (10x objective for 4T1/Ds-red2 and FEMX-1/eGFP, 40X objective for EMT6 and B16; 20x objective for all the others); right panel: Percentage of spheroid-forming cells in cancer cell lines and their fluorescent clones. B. The effect of various growth factors on the formation of spheroids by GL-261, U87Mg and FEMX-1 cells. LIF, leukemia inhibitory factor; IL-6, interleukin 6; IL-11, interleukin 11; CT-1, cardiotrophin 1; SCF, stem cell factor; EGF, epidermal growth factor; bFGF, basic fibroblast growth factor. Results were reproduced in at least three independent experiments. Results from typical experiments performed in triplicate are shown; bars, SD.