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. Author manuscript; available in PMC: 2009 Jun 9.
Published in final edited form as: Exp Cell Res. 2008 Mar 20;314(10):2110–2122. doi: 10.1016/j.yexcr.2008.03.008

Figure 3. Flow cytometric analysis of c-kit in the different phases of the cell cycle of GL-261/adh, GL-261/sph and of ex-vivo GL-261, dissociated from brain tumors of mice derived from GL-261/adh or GL-261/sph cells.

Figure 3

The supravital DNA stain Hoechst 33342 was employed for cell cycle analysis and to distinguish normal diploid brain cells from hyperdiploid GL-261 cells. P3–P5 and P6–P8 represent gates for c-kit-positive cells and c-kit-negative cells, respectively, compared with their isotypic control. P3 and P6, cells in G0/G1; P4 and P7, cells in S; P5 and P8, cells in G2-M phases of the cell cycle. N, normal brain cells.