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. 2009 Apr 28;420(Pt 1):57–65. doi: 10.1042/BJ20082397

Figure 5. In vitro dephosphorylation of purified AtPPC1 from −Pi suspension cells using the catalytic subunit of bovine PP2A.

Figure 5

Purified AtPPC1 was incubated with PP2A for the indicated times and subjected to (A) SDS/PAGE or (B) Pi-affinity PAGE [30], followed by immunoblot analysis with either anti-RcPEPC Ab [26] (25 ng of AtPPC1/lane) or the corresponding anti-pSer-11 Ab [28] (250 ng of AtPPC1/lane). ‘Phospho’ and ‘Dephospho’ denote the phosphorylated and dephosphorylated forms of p107 respectively.