. 2009 Apr 28;420(Pt 1):57–65. doi: 10.1042/BJ20082397

Table 1. Purification of PEPC from 240 g of –P_i Arabidopsis suspension cells.

Step	Volume (ml)	Activity (units)	Protein (mg)	Specific activity (units·mg⁻¹)	Purification (fold)	Yield (%)
Clarified extract	375	120	6750	0.034	1.0	100
(NH₄)₂SO₄ fractionation	126	74.3	3260	0.023	0.7	62
butyl-Sepharose	5.0^*	34.7	200	0.17	5.1	29
DEAE-Fractogel	1.2^*	46.3	17.0	2.7	80	39
Superdex-200 FPLC	5.5	40.0	2.8	14.5	450	33
Mono Q FPLC	0.7^*	28.0	1.3	22.3	660	23

*Concentrated pooled fractions.

Table 1. Purification of PEPC from 240 g of –Pi Arabidopsis suspension cells.